The ALOXE3 Polyclonal Antibody (CAB8245) is a valuable tool for researchers studying ALOXE3, an enzyme involved in the metabolism of fatty acids and skin barrier function. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it an ideal choice for Western blot applications.ALOXE3, also known as arachidonate lipoxygenase 3, plays a crucial role in the skin's lipid barrier function, which is important for protecting against environmental stressors and maintaining skin hydration.
Dysregulation of ALOXE3 has been implicated in various skin disorders, making it a promising target for dermatological research.By using the ALOXE3 Polyclonal Antibody, researchers can effectively detect and analyze ALOXE3 protein levels in different cell types, providing insights into its function and potential role in skin health. This antibody is a valuable tool for investigating the mechanisms underlying skin conditions and for developing targeted therapies that modulate ALOXE3 activity.
Product Name:
ALOXE3 Rabbit Polyclonal Antibody
SKU:
CAB8245
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ALOXE3 (NP_067641.2).
This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
59344
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALOXE3 antibody (CAB8245) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
