The ALKBH3 Polyclonal Antibody (CAB5808) is a valuable tool for researchers studying ALKBH3, a protein involved in DNA repair and oxidative stress response. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. It specifically targets the ALKBH3 protein, enabling precise detection and analysis in various cell types.ALKBH3 is known for its role in repairing DNA damage caused by alkylating agents and is also implicated in protecting cells from oxidative stress.
Research on ALKBH3 is crucial for understanding its functions in maintaining genome integrity and preventing mutations that could lead to diseases like cancer. This antibody is ideal for investigations in the fields of molecular biology, genetics, and oncology, providing insights into the mechanisms underlying DNA repair pathways and cellular responses to stress.
Product Name:
ALKBH3 Rabbit Polyclonal Antibody
SKU:
CAB5808
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human ALKBH3 (NP_631917.1).
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).
Purification Method:
Affinity purification
Gene ID:
221120
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart, using ALKBH3 Rabbit pAb (CAB5808) at 1:4000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
