The ALKBH1 Polyclonal Antibody (CAB14079) is a valuable tool for researchers studying ALKBH1, a key enzyme involved in DNA repair and RNA modification processes. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for a variety of research applications, including Western blotting and immunohistochemistry.ALKBH1, a member of the AlkB family of proteins, plays a crucial role in maintaining genome stability by repairing alkylated DNA and oxidatively damaged RNA. Dysregulation of ALKBH1 has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis.
By utilizing the ALKBH1 Polyclonal Antibody, researchers can study the expression and localization of ALKBH1 in different cell types and tissues, providing insights into its functional role in DNA and RNA repair pathways. This antibody is a valuable tool for investigations into the molecular mechanisms underlying disease pathogenesis and the development of potential therapeutic interventions targeting ALKBH1.
Product Name:
ALKBH1 Rabbit Polyclonal Antibody
SKU:
CAB14079
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
ABH; ABH1; alkB; hABH; ALKBH; ALKBH1
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion, Nucleus.
Calculated MW:
44kDa
Observed MW:
44kDa
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.
Purification Method:
Affinity purification
Gene ID:
8846
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using ALKBH1 Rabbit pAb (CAB14079) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
