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Alkaline Phosphatase Activity Assay Kit (MAES0023)

SKU:
MAES0023
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Animal Tissue, Cells
Sensitivity:
0.27 U/L
Range:
0.27-50.8 U/L
Assay Time:
30 min
Shipping:
Gel Pack
Research Area:
Enzyme Activity
€259
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Alkaline Phosphatase Activity Assay Kit
Product Code:MAES0023
Product Size:96 Assays
Sample Type:Serum, plasma, animal tissue
Assay Time:30 min
Instrument:Microplate reader
Sensitivity:0.27 U/L
Detection Range:0.27-50.8 U/L
Inter CV:4.6
Inter CV:1.2

Alkaline phosphatase (ALP) is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase and each catalytic site contains three metal ions. There are four isozymes in humans: tissue nonspecific ALP, intestinal ALP, placental ALP and genital cell ALP.

Under alkaline conditions, alkaline phosphatase catalyzes the hydrolysis of p- nitrobenzene phosphate disodium to produce p-nitrophenol and phosphoric acid. Under strong alkaline conditions, p-nitrophenol is bright yellow and has a maximum absorption peak at 405 nm. Therefore, the activity of ALP can be calculated by measuring the OD value at 405 nm.

Shelf Life:6 Months
Storage:-20°C
Shipping:Gel Pack
Research Area:Enzymes, Liver Biomarkers

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