The ALG1 Polyclonal Antibody (CAB7818) is a valuable tool for researchers studying ALG1, a protein involved in the biosynthesis of N-linked glycoproteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.ALG1 is an essential enzyme in the glycosylation pathway, playing a crucial role in the modification of proteins for proper cellular function. Dysregulation of ALG1 has been linked to various diseases, making it a promising target for therapeutic interventions. By using the ALG1 Polyclonal Antibody, researchers can investigate the expression and localization of ALG1 in different cell types, providing insights into its biological functions and potential implications in disease pathogenesis.
With its specificity and sensitivity, the ALG1 Polyclonal Antibody is a valuable asset for studies in the fields of biochemistry, cell biology, and molecular biology. By enabling precise detection and analysis of ALG1 protein, this antibody is a valuable tool for advancing our understanding of glycosylation processes and their implications in health and disease.
Product Name:
ALG1 Rabbit Polyclonal Antibody
SKU:
CAB7818
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 215-464 of human ALG1 (NP_061982.3).
Endoplasmic reticulum membrane, Single-pass type II membrane protein.
Calculated MW:
53kDa
Observed MW:
60kDa
The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.
Purification Method:
Affinity purification
Gene ID:
56052
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALG1 antibody (CAB7818) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
