The ALDH4A1 Polyclonal Antibody (CAB2595) is a crucial tool for studying ALDH4A1, a key enzyme involved in the proline metabolism pathway. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By specifically binding to the ALDH4A1 protein, researchers can accurately detect and analyze this enzyme in various cell types.ALDH4A1 is essential for converting pyrroline-5-carboxylate into ornithine, a process critical for cellular energy production and the maintenance of amino acid balance.
Dysregulation of ALDH4A1 has been implicated in various diseases, including metabolic disorders and cancer. Therefore, understanding the function and regulation of ALDH4A1 is crucial for developing targeted therapies and diagnostic tools for these conditions.This ALDH4A1 Polyclonal Antibody is a valuable asset for researchers in the fields of biochemistry, metabolism, and cancer biology, providing a reliable tool for investigating the role of ALDH4A1 in health and disease.
Product Name:
ALDH4A1 Rabbit Polyclonal Antibody
SKU:
CAB2595
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 354-563 of human ALDH4A1 (NP_003739.2).
This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
8659
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ALDH4A1 Rabbit pAb (CAB2595) at 1:6000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
