Anti-ALDH3A2 Antibody (CAB16267)

Antibody Name:	Anti-ALDH3A2 Antibody
Antibody SKU:	CAB16267
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 250-320 of human ALDH3A2 (NP_001026976.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Mouse testis, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 250-320 of human ALDH3A2 (NP_001026976.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EASL QNQI VWKI KETV KEFY GENI KESP DYER IINL RHFK RILS LLEG QKIA FGGE TDEA TRYI APTV LTD
Gene ID:	224
Uniprot:	P51648
Cellular Location:	Cytoplasmic side, Endoplasmic reticulum membrane, Single-pass membrane protein
Calculated MW:	54kDa/57kDa
Observed MW:	55kDa

Synonyms:

ALDH3A2, ALDH10, FALDH, SLS

Background:

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Mutations in the gene cause Sjogren-Larsson syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ALDH3A2: Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS). SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - histidine; Membrane protein, integral; Oxidoreductase; EC 1.2.1.3; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - beta-alanine; Lipid Metabolism - fatty acid; Amino Acid Metabolism - arginine and proline; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrial inner membrane; integral to membrane; peroxisome Molecular Function:long-chain-alcohol oxidase activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; long-chain-aldehyde dehydrogenase activity Biological Process: phytol metabolic process; epidermis development; central nervous system development; aldehyde metabolic process; sesquiterpenoid metabolic process; peripheral nervous system development Disease: Sjogren-larsson Syndrome
NCBI Summary:	Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This gene product catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acid. Mutations in the gene cause Sjogren-Larsson syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P51648
NCBI GenInfo Identifier:	1706379
NCBI Gene ID:	224
NCBI Accession:	P51648.1
UniProt Secondary Accession:	P51648,Q6I9T3, Q93011, Q96J37,
UniProt Related Accession:	P51648
Molecular Weight:	485
NCBI Full Name:	Fatty aldehyde dehydrogenase
NCBI Synonym Full Names:	aldehyde dehydrogenase 3 family, member A2
NCBI Official Symbol:	ALDH3A2
NCBI Official Synonym Symbols:	SLS; FALDH; ALDH10
NCBI Protein Information:	fatty aldehyde dehydrogenase; aldehyde dehydrogenase 10; microsomal aldehyde dehydrogenase; aldehyde dehydrogenase family 3 member A2
UniProt Protein Name:	Fatty aldehyde dehydrogenase
UniProt Synonym Protein Names:	Aldehyde dehydrogenase 10; Aldehyde dehydrogenase family 3 member A2; Microsomal aldehyde dehydrogenase
Protein Family:	Fatty aldehyde dehydrogenase
UniProt Gene Name:	ALDH3A2
UniProt Entry Name:	AL3A2_HUMAN

	Western blot analysis of extracts of various cell lines, using ALDH3A2 Rabbit pAb (CAB16267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of L929 cells using ALDH3A2 Polyclonal Antibody (CAB16267) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-ALDH3A2 Antibody (CAB19899)[KO Validated]

ALDH3A2 Antibody (PACO07685)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)