The ALDH2 Polyclonal Antibody (CAB13463) is a valuable tool for researchers studying ALDH2, an enzyme involved in alcohol metabolism and oxidative stress response. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the ALDH2 protein, this antibody allows for precise detection and analysis of ALDH2 levels in various cell types, making it an essential tool for studies in toxicology, pharmacology, and alcohol-related research.
ALDH2 is known for its crucial role in detoxifying acetaldehyde, a toxic byproduct of alcohol metabolism, and for protecting cells against oxidative damage. Research on ALDH2 is important for understanding the mechanisms of alcohol-related diseases, such as liver cirrhosis and cancer, as well as for developing targeted therapies for these conditions. By studying the activity of ALDH2, researchers can gain insights into how alcohol consumption impacts cellular function and identify potential therapeutic interventions.
Product Name:
ALDH2 Rabbit Polyclonal Antibody
SKU:
CAB13463
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human ALDH2 (NP_000681.2).
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
217
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALDH2 antibody (CAB13463) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
