The ALDH2 Rabbit Monoclonal Antibody is a valuable tool for researchers studying the ALDH2 protein, which is involved in alcohol metabolism and detoxification. This antibody, generated in rabbits, exhibits high specificity for human samples and is suitable for use in Western blotting and immunohistochemistry applications. By targeting the ALDH2 protein, this antibody enables researchers to detect and analyze ALDH2 expression in various cell types, making it an essential tool for investigations in alcohol metabolism, liver function, and drug metabolism research.
ALDH2, a key enzyme in the metabolism of acetaldehyde, plays a crucial role in alcohol-induced toxicity and alcohol-related diseases. Its genetic variants have been linked to an increased risk of alcohol-related complications, making it an important target for studies on alcoholism, liver diseases, and drug interactions. Understanding the function and regulation of ALDH2 is essential for developing interventions and treatments for individuals at risk for alcohol-related diseases.
Product Name:
ALDH2 Rabbit Monoclonal Antibody
SKU:
CAB11500
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ALDH2 (P05091).
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
217
Clone Number:
ARC0623
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALDH2 Rabbit mAb (CAB11500) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
