Aldehyde Dehydrogenase (ALDH) Fluorometric Activity Assay Kit (MAES0221)
- SKU:
- MAES0221
- Product Type:
- Assay
- Instrument:
- Fluorescence Microplate Reader
- Detection Method:
- Fluorometric
- Sample Type:
- Serum, Plasma, Animal Tissue, Cell
- Sensitivity:
- 0.06 μmol/L
- Range:
- 0.06-3 μmol/L
- Assay Time:
- 20 min
Description
| Product Name: | Aldehyde Dehydrogenase (ALDH) Fluorometric Activity Assay Kit |
| Product Code: | MAES0221 |
| Sample Type: | Serum (Plasma), Animal Tissue, Cell |
| Assay Time: | 20 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | ALDH |
| Detection Instrument: | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Detection Method: | Fluorescence method |
| Sensitivity: | 0.06 μmol/L |
| Detection Range: | 0.06–3 μmol/L |
| Inter-assay CV: | 0.084 |
| Intra-assay CV: | 0.04 |
The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, NADH reacts with the fluorescent probe to form fluorescent substance under the action of enzymes. The activity of ALDH can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Micropipettor, Centrifuge |
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