Alcohol dehydrogenase Activity Assay Kit (BA0011)

SKU:: BA0011
UPC:
Product Type:: Assay
Instrument:: Microplate Reader
Sample Type:: Biological (Plasma, Serum, Urine, Tissue, Culture Media)
Research Area:: Plant & Environmental Stress; Clinical Chemistry

€719

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Description

Description

Alcohol dehydrogenase Activity Assay Kit - Information

Assay Genie's non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Applications

For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.

Alcohol dehydrogenase Activity Assay Kit - Key Features

Fast and sensitive. Linear detection range (20 sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Alcohol dehydrogenase Activity Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 10 mL Diaphorase : 120 uL NAD Solution: 1 mL Calibrator: 1.5 mL MTT Solution: 1.5 mL Substrate: 1 mL 10% Ethanol
Kit Requires	Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader.
Method of Detection	OD565nm
Detection Limit	0.4 U/L
Samples	Biological (e.g. plasma, serum, urine, tissue and culture media)
Species	All
Protocol Length	30 min
Size	100 tests
Storage	Store all components at -20°C upon receiving
Shelf Life	6 months

More Details

ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.