The ALAS1 Rabbit Monoclonal Antibody (CAB4817) is a valuable tool for researchers investigating ALAS1, an enzyme involved in heme biosynthesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the ALAS1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it well-suited for studies in biochemistry, metabolism, and related fields.
ALAS1 is a key enzyme in the heme biosynthetic pathway, playing a crucial role in the production of heme, an essential component of hemoglobin and other proteins. Dysregulation of ALAS1 activity has been linked to various disorders, including porphyrias and other conditions affecting heme synthesis. Investigating the function and regulation of ALAS1 is important for understanding these diseases and developing potential therapeutic strategies targeting heme metabolism.
Product Name:
ALAS1 Rabbit Monoclonal Antibody
SKU:
CAB4817
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 541-640 of human ALAS1 (P13196).
This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
211
Clone Number:
ARC0239
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALAS1 Rabbit mAb (CAB4817) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
