The ALAD Polyclonal Antibody (CAB8398) is a valuable tool for researchers studying aminolevulinic acid dehydratase (ALAD), an enzyme involved in the biosynthesis of heme. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ALAD protein, this antibody enables detection and analysis in various cell types, making it ideal for investigations in areas such as biochemistry, metabolism, and hematology.ALAD is a critical enzyme in the heme biosynthetic pathway, playing a key role in the production of heme, which is essential for various biological processes including oxygen transport and energy production.
Dysregulation of ALAD has been linked to conditions such as porphyria, a group of disorders characterized by abnormal heme synthesis. Research into the function and regulation of ALAD is important for understanding the molecular mechanisms underlying these diseases and for the development of potential therapeutic interventions.Overall, the ALAD Polyclonal Antibody (CAB8398) is a reliable tool for researchers investigating the role of ALAD in heme synthesis and related pathways. Its specificity and sensitivity make it a valuable asset for studies aiming to elucidate the functions of this enzyme and its implications in various health conditions.
Product Name:
ALAD Rabbit Polyclonal Antibody
SKU:
CAB8398
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ALAD (NP_000022.3).
The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
210
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ALAD antibody (CAB8398) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
