The AKT3 Monoclonal Antibody is a vital tool for research involving the AKT3 protein, a key player in cell growth and survival pathways. This antibody, produced using hybridoma technology, is highly specific to human AKT3 and has been validated for use in various applications, including Western blot and immunohistochemistry.AKT3, a member of the AKT family of serine/threonine protein kinases, is involved in processes such as cell proliferation, apoptosis, and metabolism. Dysregulation of AKT3 has been implicated in various diseases, including cancer and neurological disorders.
By targeting AKT3 with this monoclonal antibody, researchers can study its role in cellular signaling pathways and potentially develop targeted therapies for conditions associated with AKT3 dysfunction.Overall, the AKT3 Monoclonal Antibody is a valuable tool for investigators studying the complex functions of the AKT3 protein and its implications in various disease states. Its high specificity and reliability make it an essential component for research in oncology, neuroscience, and other fields where AKT3 is a key player.
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
10000
Clone Number:
ARC57891
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using AKT3 antibody (CAB22738) at 1:60000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
