The AKT2 Polyclonal Antibody (CAB0336) is a valuable tool for research involving AKT2, a key signaling molecule in cell growth, survival, and metabolism. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the AKT2 protein, researchers can easily detect and analyze its expression in various cell types, making it an ideal choice for studies in cell biology and cancer research.AKT2, also known as protein kinase B, is a critical regulator of multiple cellular processes, including cell proliferation, survival, and glucose metabolism. Dysregulation of AKT2 signaling has been implicated in various diseases, such as cancer, diabetes, and cardiovascular disorders.
Studying the function and activity of AKT2 is essential for gaining insights into these pathological conditions and developing targeted therapies to treat them effectively.Overall, the AKT2 Polyclonal Antibody (CAB0336) serves as a valuable tool for researchers aiming to explore the role of AKT2 in cellular signaling pathways and its implications in disease development and progression. Its high specificity and sensitivity make it a reliable choice for advancing our understanding of AKT2 biology and its potential as a therapeutic target in various health conditions.
Product Name:
AKT2 Rabbit Polyclonal Antibody
SKU:
CAB0336
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human AKT2 (NP_001617.1).
Sequence:
AKEV MEHR FFLS INWQ DVVQ KKLL PPFK PQVT SEVD TRYF DDEF TAQS ITIT PPDR YDSL GLLE LDQR THFP QFSY SASI RE
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100
Synonyms:
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; AKT2
Positive Sample:
HeLa,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein.
Calculated MW:
56kDa
Observed MW:
65kDa
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
Purification Method:
Affinity purification
Gene ID:
208
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AKT2 antibody (CAB0336).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
