The AKT1S1/PRAS40 Polyclonal Antibody (CAB6238) is specifically designed for researchers studying the AKT1S1/PRAS40 protein, a key regulator of the Akt/mTOR signaling pathway involved in cell growth and survival. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.The AKT1S1/PRAS40 protein is a critical player in the Akt/mTOR pathway, impacting cell proliferation and survival through its interactions with various downstream effectors. Dysregulation of this pathway has been implicated in numerous diseases, including cancer, making the AKT1S1/PRAS40 protein a promising target for therapeutic interventions.
By using the AKT1S1/PRAS40 Polyclonal Antibody, researchers can detect and analyze the levels and activity of the AKT1S1/PRAS40 protein in a variety of cell types, allowing for insights into its role in cellular processes and disease development. This antibody is a valuable tool for studies in cancer research, cell biology, and signal transduction pathways, offering new opportunities for understanding the mechanisms underlying disease progression and potential therapeutic interventions.
Product Name:
AKT1S1/PRAS40 Polyclonal Antibody
SKU:
CAB6238
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-256 of human AKT1S1/PRAS40 (NP_001092103.1).
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
Purification Method:
Affinity purification
Gene ID:
84335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AKT1S1/PRAS40 Rabbit pAb (CAB6238) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
