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AKT1S1/PRAS40 Polyclonal Antibody (CAB21686)

SKU:
CAB21686
$155
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Description

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Product Name:AKT1S1/PRAS40 Polyclonal Antibody
SKU:CAB21686
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-256 of human AKT1S1/PRAS40 (NP_001092103.1).
Sequence:STDD GSLS EETP AGPP TCSV PPAS ALPT QQYA KSLP VSVP VWGF KEKR TEAR SSDE ENGP PSSP DLDR IAAS MRAL VLRE AEDT QVFG DLPR PRLN TSDF QKLK RKY
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:Lobe; PRAS40; AKT1S1/PRAS40
Positive Sample:PC-3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, cytosol.
Calculated MW:27kDa
Observed MW:40kDa

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).

Purification Method:Affinity purification
Gene ID:84335
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from PC-3 cells using AKT1S1/PRAS40 Rabbit pAb(CAB21686) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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