The AKT1S1/PRAS40 Polyclonal Antibody (CAB21686) is a vital tool for researchers studying the AKT1S1/PRAS40 protein, which plays a key role in the PI3K/AKT signaling pathway, regulating cell growth, survival, and metabolism. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for Western blot applications.AKT1S1/PRAS40, a known substrate of AKT, functions as a key regulator of mTORC1 activity, influencing cell growth and protein synthesis. Dysregulation of the AKT1S1/PRAS40 pathway has been implicated in various diseases, including cancer, diabetes, and neurological disorders, making it a promising target for therapeutic intervention.
By enabling precise detection and analysis of AKT1S1/PRAS40 expression levels in different cell types, the CAB21686 antibody facilitates investigations into the molecular mechanisms underlying disease pathology and the development of novel treatments targeting the PI3K/AKT pathway. Researchers in fields such as oncology, metabolism, and neurobiology will benefit from utilizing this versatile and reliable antibody in their studies.
Product Name:
AKT1S1/PRAS40 Polyclonal Antibody
SKU:
CAB21686
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-256 of human AKT1S1/PRAS40 (NP_001092103.1).
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
Purification Method:
Affinity purification
Gene ID:
84335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-3 cells using AKT1S1/PRAS40 Rabbit pAb(CAB21686) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
