The AKT1S1 (PRAS40) Monoclonal Antibody is a crucial tool for researchers studying the AKT1S1 protein, which plays a key role in the PI3K-AKT signaling pathway. This antibody, developed using advanced monoclonal antibody technology, specifically targets the AKT1S1 protein, allowing for precise detection and analysis in a variety of experimental settings.AKT1S1, also known as PRAS40, is a critical regulator of cell growth, proliferation, and survival, making it a key player in processes like tumorigenesis and metabolic regulation. By targeting the AKT1S1 protein, researchers can gain valuable insights into the mechanisms underlying these processes and potential therapeutic targets for diseases like cancer and metabolic disorders.
The AKT1S1 (PRAS40) Monoclonal Antibody is highly specific to human samples and has been validated for use in techniques like Western blot and immunohistochemistry, providing reliable results in diverse experimental contexts. Its exceptional reactivity and specificity make it an indispensable tool for studies in cancer biology, cell signaling, and drug development efforts aimed at targeting the PI3K-AKT pathway.
Product Name:
AKT1S1/PRAS40 Monoclonal Antibody
SKU:
CAB3391
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-256 of human AKT1S1/PRAS40 (Q96B36).
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
Purification Method:
Affinity purification
Gene ID:
84335
Clone Number:
ARC1965
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AKT1S1/AKT1S1/PRAS40 Rabbit mAb (CAB3391) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
