AKT1S1/PRAS40 Monoclonal Antibody (CAB3391)
- SKU:
- CAB3391
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | AKT1S1/PRAS40 Monoclonal Antibody |
| SKU: | CAB3391 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-256 of human AKT1S1/PRAS40 (Q96B36). |
| Sequence: | STDD GSLS EETP AGPP TCSV PPAS ALPT QQYA KSLP VSVP VWGF KEKR TEAR SSDE ENGP PSSP DLDR IAAS MRAL VLRE AEDT QVFG DLPR PRLN TSDF QKLK RKY |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | Lobe; PRAS40; AKT1S1/PRAS40 |
| Positive Sample: | HeLa,MCF7,NIH/3T3,RD |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, cytosol. |
| Calculated MW: | 27kDa |
| Observed MW: | 40kDa |
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
| Purification Method: | Affinity purification |
| Gene ID: | 84335 |
| Clone Number: | ARC1965 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using AKT1S1/AKT1S1/PRAS40 Rabbit mAb (CAB3391) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min. |
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