The AKAP5 Polyclonal Antibody (CAB14091) is a valuable tool for researchers studying AKAP5, a scaffolding protein that plays a crucial role in signal transduction and cell signaling pathways. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the AKAP5 protein, this antibody allows for the detection and analysis of AKAP5 expression in various cell types, making it an ideal choice for immunology and cancer research studies.AKAP5, also known as A-kinase anchor protein 5, is involved in the organization of molecular complexes that regulate the localization and activity of protein kinase A (PKA) in cells.
Its function in modulating intracellular signaling cascades makes it a key target for research into various diseases, including cancer and cardiovascular disorders. By elucidating the role of AKAP5 in these pathologies, researchers can gain insights that may lead to the development of novel therapeutic strategies aimed at targeting AKAP5-mediated signaling pathways.Overall, the AKAP5 Polyclonal Antibody (CAB14091) provides researchers with a reliable tool for investigating the biological functions and signaling mechanisms associated with AKAP5, ultimately contributing to a better understanding of its role in health and disease.
Product Name:
AKAP5 Rabbit Polyclonal Antibody
SKU:
CAB14091
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3).
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT.
Purification Method:
Affinity purification
Gene ID:
9495
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using AKAP5 Rabbit pAb (CAB14091) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
