The AKAP4 Polyclonal Antibody (CAB14813) is a valuable tool for researchers studying AKAP4, a key protein involved in sperm flagellar formation and function. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various experimental applications, including Western blotting. By binding specifically to AKAP4, this antibody enables precise detection and analysis of the protein in different cell types, making it a versatile tool for studies in reproductive biology and fertility research.
AKAP4, also known as A-kinase anchor protein 4, plays a crucial role in sperm motility and fertilization by anchoring protein kinase A (PKA) to the flagella, thereby regulating cAMP signaling and flagellar movement. Understanding the function and regulation of AKAP4 is essential for gaining insights into male infertility, reproductive disorders, and potential targets for contraceptive development. By using the AKAP4 Polyclonal Antibody, researchers can uncover new findings and advancements in the field of reproductive science.
Product Name:
AKAP4 Rabbit Polyclonal Antibody
SKU:
CAB14813
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2).
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
8852
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using AKAP4 antibody (CAB14813) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
