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Ajuba Polyclonal Antibody (CAB21760)

SKU:
CAB21760
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €529
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Ajuba Polyclonal Antibody
SKU:CAB21760
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 201-290 of human Ajuba (NP_116265.1).
Sequence:PSAY PELH AALD RLYA QRPA GFGC QESR HSYP PALG SPGA LAGA GVGA AGPL ERRG AQPG RHSV TGYG DCAV GARY QDEL TALL RLTV GT
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:JUB; ba
Positive Sample:HepG2,Rat kidney
Conjugate:Unconjugated
Cellular Localization:adherens junction, cell-cell junction, cytosol, Golgi apparatus, microtubule organizing center, nucleoplasm, nucleus, P-body, plasma membrane
Calculated MW:57kDa
Observed MW:55kDa

Enables alpha-catenin binding activity and transcription corepressor activity. Involved in several processes, including negative regulation of hippo signaling; positive regulation of gene silencing by miRNA; and regulation of cellular response to hypoxia. Acts upstream of or within gene silencing by miRNA and positive regulation of protein-containing complex assembly. Located in several cellular components, including Golgi apparatus; P-body; and nucleoplasm.

Purification Method:Affinity purification
Gene ID:84962
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of HepG2, using Ajuba Rabbit pAb antibody (CAB21760) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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