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Anti-AIF Antibody (CAB19536)

SKU:
CAB19536
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
€139 - €419
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Description

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Antibody Name:Anti-AIF Antibody
Antibody SKU:CAB19536
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human AIF.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, U-251MG, HeLa, Mouse brain, Mouse kidney, Mouse heart, Rat heart, Rat liver
Immunogen:A synthesized peptide derived from human AIF.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9131
Uniprot:O95831
Cellular Location:
Calculated MW:67kDa
Observed MW:67KDa
Anti-AIF Antibody (CAB19536)Western blot - AIF Rabbit mAb (CAB19536)
Synonyms:AIF, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, AIFM1
Background:This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]
UniProt Protein Function:PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; EC 1.-.-.-; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; nucleus

Molecular Function:NAD(P)H oxidase activity; oxidoreductase activity, acting on NADH or NADPH; protein binding

Biological Process: apoptosis; caspase activation; chromosome condensation; positive regulation of apoptosis

Disease: Deafness, X-linked 5

NCBI Summary:This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]
UniProt Code:O95831
NCBI GenInfo Identifier:13431764
NCBI Gene ID:9131
NCBI Accession:O95831.1
UniProt Secondary Accession:O95831,Q1L6K4, Q1L6K6, Q2QKE4, Q5JUZ7, Q6I9X6, Q9Y3I3 Q9Y3I4, A4QPB4, B1ALN1, B2RB08, D3DTE9,
UniProt Related Accession:O95831
Molecular Weight:26,033 Da
NCBI Full Name:Apoptosis-inducing factor 1, mitochondrial
NCBI Synonym Full Names:apoptosis inducing factor mitochondria associated 1
NCBI Official Symbol:AIFM1  
NCBI Official Synonym Symbols:AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6  
NCBI Protein Information:apoptosis-inducing factor 1, mitochondrial
UniProt Protein Name:Apoptosis-inducing factor 1, mitochondrial
UniProt Synonym Protein Names:Programmed cell death protein 8
Protein Family:Putative apoptosis-inducing factor
UniProt Gene Name:AIFM1  

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