The AIF Rabbit Monoclonal Antibody (CAB19536) is a valuable tool for researchers studying apoptosis induction factor (AIF), a mitochondrial protein involved in programmed cell death. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting AIF in human samples, making it suitable for a range of applications including Western blotting and immunohistochemistry.AIF is a multifunctional protein that plays a crucial role in apoptotic pathways, translocating from the mitochondria to the nucleus to induce chromatin condensation and DNA fragmentation.
Dysregulation of AIF has been implicated in various diseases, including neurodegenerative disorders and cancer, making it a promising target for therapeutic interventions.By utilizing the AIF Rabbit Monoclonal Antibody, researchers can effectively study the expression and localization of AIF in different cellular contexts, providing valuable insights into its role in cell death and disease progression. This antibody is a valuable addition to any laboratory conducting research in the fields of cell biology, neuroscience, and oncology.
Product Name:
AIF Rabbit Monoclonal Antibody
SKU:
CAB19536
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 450-550 of human AIF (O95831).
Sequence:
RRVE HHDH AVVS GRLA GENM TGAA KPYW HQSM FWSD LGPD VGYE AIGL VDSS LPTV GVFA KATA QDNP KSAT EQSG TGIR SESE TESE ASEI TIPP STPA V
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
Purification Method:
Affinity purification
Gene ID:
9131
Clone Number:
ARC0015
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using AIF Rabbit mAb (CAB19536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
