The AIF Polyclonal Antibody (ABC12345) is a valuable tool for researchers studying AIF, a key protein involved in apoptosis and cell death regulation. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in various applications such as Western blot and immunofluorescence.AIF, or apoptosis-inducing factor, is a mitochondrial protein that plays a crucial role in the regulation of programmed cell death. Dysregulation of AIF has been implicated in various diseases, including neurodegenerative disorders and cancers. By using this antibody to detect and analyze AIF expression in different cell types, researchers can gain valuable insights into the molecular mechanisms underlying these diseases.
Furthermore, the AIF Polyclonal Antibody is an essential tool for investigating the potential therapeutic targeting of AIF in disease treatment. Understanding the role of AIF in cell death pathways can lead to the development of novel therapies for conditions where apoptosis is dysregulated. This antibody is a valuable resource for researchers in the fields of cell biology, molecular biology, and cancer research.
Product Name:
AIF Polyclonal Antibody
SKU:
CAB0811
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1).
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
Purification Method:
Affinity purification
Gene ID:
9131
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using AIF Rabbit pAb (CAB0811).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
