The AHCY Polyclonal Antibody (CAB5300) is a valuable tool for researchers studying AHCY, an essential enzyme involved in the methionine cycle and critical for maintaining cellular methylation potential. This antibody, generated in rabbits, is highly specific to human AHCY and has been validated for use in Western blot applications. It detects the AHCY protein, allowing for precise analysis in various cell types, making it an ideal choice for studies in biochemistry and molecular biology.
AHCY, also known as S-adenosylhomocysteine hydrolase, plays a crucial role in regulating cellular methylation processes by catalyzing the hydrolysis of S-adenosylhomocysteine (SAH). Dysregulation of AHCY has been implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions. Research into the functions and mechanisms of AHCY is essential for developing targeted therapies that exploit its role in these pathologies.
Product Name:
AHCY Rabbit Polyclonal Antibody
SKU:
CAB5300
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 253-432 of human AHCY (NP_000678.1).
S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
191
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AHCY antibody (CAB5300) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
