The AGRN Polyclonal Antibody (CAB17320) is a valuable tool for researching agrin, a protein found in the nervous system that is crucial for the formation and maintenance of neuromuscular junctions. This polyclonal antibody, generated in rabbits, has high reactivity with human samples and is validated for use in immunofluorescence and immunohistochemistry applications.Agrin is known for its role in organizing postsynaptic differentiation at the neuromuscular junction, a critical site for communication between nerves and muscles. By targeting agrin with this antibody, researchers can visualize and study the distribution and function of agrin in different tissues and cell types, providing insights into its involvement in neuromuscular development and function.
The AGRN Polyclonal Antibody is an essential tool for studies in neuroscience, muscle biology, and developmental biology, shedding light on the intricate mechanisms underlying neuromuscular junction formation and maintenance. Researchers interested in understanding the role of agrin in neuromuscular disorders, muscle development, and synaptic plasticity will find this antibody invaluable for their investigations.
Product Name:
AGRN Rabbit Polyclonal Antibody
SKU:
CAB17320
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-350 of human AGRN (NP_940978.2).
This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
375790
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AGRN Rabbit pAb (CAB17320) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
