Additionally, the CD300A Polyclonal Antibody (CAB10006) from AssayGenie is produced using state-of-the-art technology to ensure high specificity and sensitivity. It has been rigorously tested and validated to provide reliable and reproducible results in experimental settings. Researchers can trust this antibody to deliver accurate and precise data for their studies on CD300A function and signaling pathways.Furthermore, with its affinity for human samples, this antibody offers versatility for a wide range of applications, including immunofluorescence, immunohistochemistry, and flow cytometry. Its exceptional performance in Western blot assays makes it a valuable tool for investigating CD300A expression levels and protein interactions.
Researchers can gain valuable insights into the role of CD300A in immune regulation and its potential as a therapeutic target for various diseases.In summary, the CD300A Polyclonal Antibody (CAB10006) from AssayGenie is a high-quality reagent that provides reliable and accurate results for studying CD300A in immunology and cancer research. Its specificity, sensitivity, and validation make it an essential tool for researchers seeking to unravel the complex mechanisms of immune regulation and potentially develop novel therapeutic strategies.
Antibody Name:
AGO4 Antibody (PACO19149)
Antibody SKU:
PACO19149
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide of human AGO4
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Raji cells, 293T cells, PC3 cells, Primary antibody: PACO19149(AGO4 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
Background:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1.
Synonyms:
argonaute RISC catalytic component 4
UniProt Protein Function:
AGO4: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV). Interacts with EIF4B, IMP8, PRMT5, TNRC6A and TNRC6B. Belongs to the argonaute family. Ago subfamily.Protein type: Translation; Translation initiationChromosomal Location of Human Ortholog: 1p34Cellular Component: cytoplasm; cytosol; membraneMolecular Function: double-stranded RNA binding; miRNA binding; protein binding; single-stranded RNA bindingBiological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; mRNA catabolic process; phosphoinositide-mediated signaling; pre-microRNA processing; RNA secondary structure unwinding; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1. [provided by RefSeq, Jul 2008]
