The AGL Polyclonal Antibody (CAB10051) is a valuable tool for researchers studying the AGL protein, an important enzyme that plays a key role in glycogen metabolism. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the AGL protein, allowing for detection and analysis in various cell types, making it an essential component for studies in biochemistry and metabolism research.The AGL enzyme is essential for breaking down glycogen into glucose, a crucial process in maintaining energy levels in the body. Dysregulation of AGL function is linked to glycogen storage diseases, a group of rare genetic disorders that disrupt glycogen metabolism and can lead to severe health complications.
By investigating the activity of AGL using this polyclonal antibody, researchers can gain insights into the molecular mechanisms underlying these diseases and develop potential therapeutic interventions.Overall, the AGL Polyclonal Antibody (CAB10051) is a powerful tool for studying the role of the AGL enzyme in glycogen metabolism and its implications for human health. Its high specificity and sensitivity make it ideal for a wide range of research applications, including studying metabolic disorders, exploring potential drug targets, and advancing our understanding of cellular metabolism.
Product Name:
AGL Rabbit Polyclonal Antibody
SKU:
CAB10051
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1233-1532 of human AGL (NP_000633.2).
This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
178
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AGL antibody (CAB10051) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
