The Afp Polyclonal Antibody (CAB16750) is a specialized research tool for studying Alpha-fetoprotein (AFP), a protein that is commonly used as a biomarker for liver cancer and certain fetal abnormalities. This antibody, generated in rabbits, is specifically designed for high reactivity with human samples and has been validated for use in various applications, including Western blot.AFP is a glycoprotein that is typically produced by the liver during fetal development, and elevated levels of AFP in adult blood can indicate certain medical conditions, particularly liver cancer. The Afp Polyclonal Antibody binds specifically to AFP, allowing for accurate detection and analysis in research studies focusing on liver cancer, fetal development, and related conditions.
Research into AFP and its role as a biomarker is crucial for improving early diagnosis and treatment of liver cancer, as well as understanding fetal development and potential abnormalities. By utilizing the Afp Polyclonal Antibody, researchers can further explore the functions and implications of AFP in various biological contexts, paving the way for advancements in clinical diagnostics and therapeutic interventions.
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Purification Method:
Affinity purification
Gene ID:
174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Alpha-Fetoprotein (AFP) Rabbit pAb (CAB16750) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
