AFG1L Antibody (PACO38066)
- SKU:
- PACO38066
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | AFG1L Antibody (PACO38066) |
| Antibody SKU: | PACO38066 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human AFG1-like ATPase protein (14-313AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: A549 whole cell lysate, Rat kidney tissue. All lanes: AFG1L antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55 kDa. Observed band size: 55 kDa. |
 | IHC image of PACO38066 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO38066 diluted at 1:600 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Synonyms: | AFG1-like ATPase (Lactation elevated protein 1) (EC 3.6.-.-) (Protein AFG1 homolog), AFG1L, AFG1 LACE1 |
| UniProt Protein Function: | Putative mitochondrial ATPase. Plays a role in mitochondrial morphology and mitochondrial protein metabolism. Promotes degradation of excess nuclear-encoded complex IV subunits (COX4I1, COX5A and COX6A1) and normal activity of complexes III and IV of the respiratory chain (PubMed:26759378, PubMed:27323408). Mediates mitochondrial translocation of TP53 and its transcription-independent apoptosis in response to genotoxic stress (PubMed:27323408). |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a mitochondrial integral membrane protein that plays a role in mitochondrial protein homeostasis. The protein contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. It functions to mediate the degradation of nuclear-encoded complex IV subunits. Two conserved estrogen receptor binding sites are located within 2.5 kb of this gene. Polymorphisms in this gene have been associated with bipolar disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2016] |
| UniProt Code: | Q8WV93 |
| NCBI GenInfo Identifier: | 17391051 |
| NCBI Gene ID: | 246269 |
| NCBI Accession: | BC018445 |
| UniProt Secondary Accession: | Q8WV93,Q8N6A3 |
| UniProt Related Accession: | Q8WV93 |
| Molecular Weight: | 54,845 Da |
| NCBI Full Name: | Homo sapiens lactation elevated 1, mRNA |
| NCBI Synonym Full Names: | AFG1 like ATPase |
| NCBI Official Symbol: | AFG1LÂ Â |
| NCBI Official Synonym Symbols: | AFG1; LACE1; c222389Â Â |
| NCBI Protein Information: | AFG1-like ATPase |
| UniProt Protein Name: | AFG1-like ATPase |
| UniProt Synonym Protein Names: | Lactation elevated protein 1 |
| Protein Family: | Lactation elevated protein |
| UniProt Gene Name: | AFG1LÂ Â |
| UniProt Entry Name: |