The AFF4 Polyclonal Antibody (CAB4644) is a valuable tool for researchers studying the transcriptional regulator AFF4, which plays a key role in gene expression regulation and transcriptional elongation. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By targeting the AFF4 protein, researchers can effectively analyze and study its functions in various cell types, aiding in research related to gene transcription, epigenetics, and developmental biology.
AFF4 is a vital component of the super elongation complex (SEC) and is involved in promoting transcriptional elongation of RNA polymerase II. Dysregulation of AFF4 has been linked to various diseases, including cancer and developmental disorders. Understanding the mechanisms by which AFF4 operates in gene transcription can provide insights into disease progression and potential therapeutic targets. The AFF4 Polyclonal Antibody is a valuable tool for unraveling the complex functions of AFF4 and its implications in human health and disease.
Product Name:
AFF4 Rabbit Polyclonal Antibody
SKU:
CAB4644
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human AFF4 (NP_055238.1).
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23).
Purification Method:
Affinity purification
Gene ID:
27125
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using AFF4 antibody (CAB4644) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (AbGn00020)._Exposure time: 30s.
