The AFAP1 Antibody (CAB3478) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2765127 Gene ID 60312 Swiss Prot Synonym AFAP; AFAP110; AFAP-110; AFAP1
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AFAP1 Antibody
SKU:
CAB3478
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AFAP, AFAP110, AFAP-110, AFAP1
Positive Sample:
22Rv1, A-549, HeLa, U-251MG, HepG2, Mouse liver, Rat liver
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
81kDa
Observed MW:
81kDa
The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. RRID AB_2765127 Gene ID 60312 Swiss Prot Synonym AFAP; AFAP110; AFAP-110; AFAP1
Purification Method:
Affinity purification
Gene ID:
60312
RRID:
AB_2765127
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AFAP1 Rabbit pAb (CAB3478) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
