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Low Endotoxin Purified Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114] (AGEL0494)

SKU:
AGEL0494
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
€229 - €1,219
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Description

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Low Endotoxin Purified Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]

The AF-Le Purified Mouse MHC II (I-A/I-E) Monoclonal Antibody (M5/114) is a powerful tool for researchers investigating the major histocompatibility complex (MHC) II molecules in mice. This monoclonal antibody, designated AGEL0494 and derived from hybridoma cells, specifically targets the I-A and I-E subtypes of MHC II, essential components of the immune system involved in antigen presentation to T cells.MHC II molecules are crucial for immune recognition and activation, playing a key role in adaptive immune responses. The M5/114 antibody is meticulously purified to provide high specificity and sensitivity in applications such as flow cytometry, immunoprecipitation, and immunohistochemistry. By binding to the I-A and I-E regions of MHC II, this antibody facilitates the identification and characterization of MHC II-expressing cells in mouse models, making it an invaluable asset for studies in immunology, infection, transplantation, and autoimmune diseases.

Researchers exploring the intricacies of immune responses, antigen presentation, and T cell activation in murine models will benefit from the precision and reliability of the AF-Le Purified Mouse MHC II (I-A/I-E) Monoclonal Antibody (M5/114). Its specificity, sensitivity, and suitability for various experimental techniques make it an essential reagent for unraveling the complexities of the immune system and its implications for health and disease.

Product Title:Low Endotoxin Purified Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]
SKU:AGEL0494
Size:50µg, 500µg, 1mg
Clone No:M5/114
Synonyms:H2-Ab1/Eb1;Major histocompatibility protein class II beta chain;MHC class II H2-IA-beta-psi;I-E beta MHC class II;MHC class II;
Host Species:Rat
Reactivity:Mouse
Application:FCM;Block;FA
Isotype:Rat IgG2b, κ
Isotype Control:AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2]
Form:Liquid
Conjugation:None (AF/LE)
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:P14483 O78196
Gene ID:14961,14969
Storage Buffer:0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

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