Low Endotoxin Purified Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1] (AGEL1400)
- SKU:
- AGEL1400
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Mouse
- Applications:
- FC
Frequently bought together:
Description
Product Title: | Low Endotoxin Purified Anti-Mouse CD45R/B220 Antibody [RA3.3A 1/6.1] |
SKU: | AGEL1400 |
Size: | 50µg, 500µg, 1mg |
Clone No: | RA3.3A 1/6.1 |
Synonyms: | B220; |
Host Species: | Rat |
Reactivity: | Mouse |
Application: | FCM;Cell Sep-Neg;Depletion |
Isotype: | Rat IgM, κ |
Isotype Control: | AF/LE Purified Rat IgM, κ Isotype Control[RTK2118] |
Form: | Liquid |
Conjugation: | None (AF/LE) |
Recommended Use: | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Gene ID: | 19264,5788 |
Storage Buffer: | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Stability & Storage: | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Background: | CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity. |