The AF-le purified anti-mouse CD54 Antibody (YN1/1.7.4) is a highly specific and reliable tool for research involving the CD54 protein, also known as intercellular adhesion molecule 1 (ICAM-1). This antibody is designed for use in a variety of experimental applications, including flow cytometry, immunohistochemistry, and functional assays.CD54/ICAM-1 is a cell surface glycoprotein involved in the immune response and inflammation. It plays a key role in cell adhesion and signaling processes, facilitating interactions between immune cells and other cell types. The AF-le purified anti-mouse CD54 Antibody (YN1/1.7.4) is raised in mice and exhibits high sensitivity and specificity for detecting CD54 expression in mouse samples.
Researchers in immunology, cell biology, and related fields will find this antibody invaluable for studying the functions and regulation of CD54/ICAM-1 in various biological contexts. By enabling precise detection and analysis of CD54, this antibody contributes to advancing our understanding of immune cell interactions, inflammatory processes, and potential therapeutic targets for immune-related disorders. Trust the AF-le purified anti-mouse CD54 Antibody (YN1/1.7.4) for accurate and reproducible results in your research endeavors.
AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2]
Form:
Liquid
Conjugation:
None (AF/LE)
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Swissprot:
P13597
Gene ID:
15894
Storage Buffer:
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.