The ADMA (Asymmetric Dimethylarginine) ELISA Kit is specially designed for the precise measurement of ADMA levels in biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.ADMA is an important metabolite that inhibits nitric oxide production and plays a role in cardiovascular diseases, kidney dysfunction, and other health conditions.
By detecting ADMA levels, researchers can better understand the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the ADMA ELISA Kit from Assay Genie provides researchers with a valuable tool for studying the role of ADMA in various pathological conditions and developing strategies for disease diagnosis and treatment.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADMA. During the reaction, ADMA in the sample or standard competes with a fixed amount of ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ADMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
92-102
99-116
Average (%)
104
97
106
1:4
Range (%)
87-100
89-103
96-109
Average (%)
93
95
103
1:8
Range (%)
90-103
93-107
96-111
Average (%)
96
99
102
1:16
Range (%)
85-100
87-103
93-107
Average (%)
92
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.2
120.8
371.6
50.5
121.3
371.7
Standard deviation
3.7
6.3
19.3
3.4
5.9
19.7
C V (%)
6.95
5.22
5.19
6.73
4.86
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ADMA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ADMA in samples. No significant cross-reactivity or interference between ADMA and analogues was observed.
