Adipolysis Assay Kit (BA0076)

SKU:

BA0076

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Culture Media

Research Area:

Lipid Metabolism

Diabetes & Obesity

High-Throughput Screening

Description

Description

Adipolysis Assay Kit - Information

Assay Genie's adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.

Applications

For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.

Adipolysis Assay Kit - Key Features

Sensitive and accurate. Use as little as 10 samples. Linear detection range in 96-well plate: 0.92 to 100 ug/mL (10 to 1000 ) glycerol for colorimetric assays and 0.2 to 5 ug/mL for fluorimetric assays.
Rapid and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates.

Adipolysis Assay Kit -Data Sheet
Kit Includes	Assay Buffer: 24 mL Enzyme Mix: 500 mL ATP: 250 mL Dye Reagent: 220 mL Standard: 100 mL 100 mM Glycerol
Kit Requires	Pipeting devices, centrifuge tubes, appropriate 96- or 384-well plates and plate reader
Method of Detection	OD570nm, or FL530/585nm
Detection Limit	0.92 ug/mL
Samples	Cell culture media
Species	All
Protocol Length	20 min
Size	200 tests
Storage	Store Assay Buffer at 4°C and other reagents at -20°C
Shelf Life	12 months

More Details

Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.