The ADH1A Polyclonal Antibody (CAB14701) is a vital tool for researchers investigating the role of ADH1A, an alcohol dehydrogenase enzyme that plays a crucial role in alcohol metabolism and detoxification. This antibody, generated in rabbits, exhibits high specificity for human samples and has been extensively validated for use in Western blot applications. By binding to the ADH1A protein, this antibody enables precise detection and analysis in a variety of cell types, making it an indispensable asset for studies in biochemistry, toxicology, and pharmacology.
ADH1A is an essential enzyme involved in the conversion of ethanol to acetaldehyde, a key step in alcohol metabolism. Dysregulation of ADH1A activity has been linked to alcohol-related liver diseases, as well as the metabolism of certain drugs and xenobiotics. Research into the function and regulation of ADH1A is crucial for understanding the mechanisms underlying alcohol dependence and toxicity, as well as for developing strategies to mitigate the harmful effects of alcohol and related substances on the body.
Product Name:
Alcohol Dehydrogenase Rabbit Polyclonal Antibody
SKU:
CAB14701
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-300 of human Alcohol Dehydrogenase (NP_000658.1).
This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.
Purification Method:
Affinity purification
Gene ID:
124
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Alcohol Dehydrogenase Rabbit pAb (CAB14701) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
