The ADAM10 Polyclonal Antibody (CAB10438) is a crucial tool for researchers investigating ADAM10, a key protein involved in the regulation of cell signaling, cell adhesion, and proteolysis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ADAM10 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in cell biology and cancer research.
ADAM10, also known as a disintegrin and metalloproteinase domain-containing protein 10, plays a critical role in various cellular processes such as cell proliferation, migration, and apoptosis. Its involvement in signal transduction pathways and cell surface protein shedding makes it a highly promising target for research into diseases like cancer, Alzheimer's disease, and inflammatory disorders. Understanding the function of ADAM10 is crucial for developing targeted therapies that manipulate its activity in disease contexts.
Product Name:
ADAM10 Rabbit Polyclonal Antibody
SKU:
CAB10438
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 218-433 of human ADAM10 (NP_001101.1).
Cell membrane, Endomembrane system, Single-pass type I membrane protein.
Calculated MW:
84kDa
Observed MW:
90kDa
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
Purification Method:
Affinity purification
Gene ID:
102
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of RAW264.7, using ADAM10 antibody (CAB10438) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 20s.
