The ADA Polyclonal Antibody (CAB13910) is a valuable tool for research involving adenosine deaminase (ADA), an enzyme essential for purine metabolism and immune function. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding specifically to ADA, this antibody enables the detection and analysis of ADA protein levels in different cell types, making it an ideal choice for studies in immunology, genetics, and metabolic disorders.
ADA plays a crucial role in the conversion of adenosine to inosine, a process that regulates cellular levels of adenosine and influences immune responses. Dysregulation of ADA activity has been implicated in various diseases, including immunodeficiency disorders and cancers. By studying ADA expression and function using this antibody, researchers can gain insights into the pathophysiology of these conditions and potentially identify new therapeutic targets for intervention.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Adenosine Deaminase (Adenosine Deaminase (ADA)) (NP_000013.2).
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.
Purification Method:
Affinity purification
Gene ID:
100
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Adenosine Deaminase (ADA) Rabbit pAb (CAB13910) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
