The ACY1 Polyclonal Antibody (CAB13458) is a valuable tool for researchers studying ACY1, an enzyme involved in amino acid metabolism and urea cycle regulation. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By specifically binding to the ACY1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in biochemistry and metabolic research.ACY1 plays a crucial role in the breakdown of amino acids and the detoxification of ammonia, making it essential for maintaining proper metabolic function.
Dysregulation of ACY1 has been linked to metabolic disorders and urea cycle defects, highlighting the importance of studying this enzyme in both normal and pathological conditions. The ACY1 Polyclonal Antibody is a valuable tool for uncovering the role of ACY1 in various cellular processes and may lead to insights for potential therapeutic interventions in metabolic diseases.
Product Name:
ACY1 Rabbit Polyclonal Antibody
SKU:
CAB13458
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-408 of human ACY1 (NP_001185824.1).
This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.
Purification Method:
Affinity purification
Gene ID:
95
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACY1 Rabbit pAb (CAB13458) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
