The ACTR3B Polyclonal Antibody (CAB7824) is a valuable tool for researchers studying ACTR3B, a key component of the Arp2/3 complex involved in actin nucleation and cytoskeleton remodeling. This antibody, produced in rabbits, shows high specificity for human samples and has been validated for use in Western blot applications. By targeting the ACTR3B protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in cell biology and cancer research.
ACTR3B plays a crucial role in cell motility, adhesion, and shape maintenance by facilitating the formation of branched actin networks. Dysregulation of ACTR3B expression has been implicated in various diseases, including cancer, where altered cytoskeleton dynamics contribute to tumor progression and metastasis. Understanding the function of ACTR3B is essential for uncovering its role in these pathological processes and developing targeted therapies to combat them.
Product Name:
ACTR3B Rabbit Polyclonal Antibody
SKU:
CAB7824
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 189-418 of human ACTR3B (NP_065178.1).
This gene encodes a member of the actin-related proteins (ARP), which form multiprotein complexes and share 35-55% amino acid identity with conventional actin. The protein encoded by this gene may have a regulatory role in the actin cytoskeleton and induce cell-shape change and motility. Pseudogenes of this gene are located on chromosomes 2, 4, 10, 16, 22 and Y. Alternative splicing results in multiple transcript variants and protein isoforms.
Purification Method:
Affinity purification
Gene ID:
57180
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ACTR3B antibody (CAB7824) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
