The ACTN4 Polyclonal Antibody (CAB16310) is a valuable tool for researchers studying ACTN4, a key protein involved in cell structure and motility. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding specifically to the ACTN4 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential reagent for studies in cell biology, cancer research, and beyond.ACTN4, also known as alpha-actinin-4, is a crucial component of the cytoskeleton and is involved in cell adhesion and migration.
Dysregulation of ACTN4 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions. Therefore, understanding the function and regulation of ACTN4 is essential for unraveling its role in disease pathogenesis and potentially identifying novel therapeutic avenues. The ACTN4 Polyclonal Antibody (CAB16310) is a reliable tool for researchers aiming to delve deeper into the mechanisms underlying ACTN4-related processes and diseases.
Product Name:
α-Actinin-4 Rabbit Polyclonal Antibody
SKU:
CAB16310
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 570-650 of human α-Actinin-4 (NP_004915.2).
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
Purification Method:
Affinity purification
Gene ID:
81
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using α-Actinin-4 Rabbit pAb (CAB16310) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
