The ACTN3 Polyclonal Antibody (CAB12864) is a valuable tool for researchers studying the ACTN3 gene, which encodes the alpha-actinin-3 protein involved in muscle function and performance. This antibody, generated in rabbits, is highly specific to human ACTN3 samples and is validated for use in Western blot applications. By binding to the ACTN3 protein, the antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in muscle biology, sports science, and exercise physiology.ACTN3 is known as the "sprinter gene" due to its association with fast-twitch muscle fibers and athletic performance in sprinting and power sports.
Understanding the role of ACTN3 in muscle contraction and performance can provide insights into muscle-related disorders, sports performance optimization, and personalized training programs. The ACTN3 Polyclonal Antibody is a valuable tool for researchers aiming to uncover the mechanisms behind muscle function and performance, ultimately contributing to advancements in sports medicine and exercise science.
Product Name:
ACTN3 Rabbit Polyclonal Antibody
SKU:
CAB12864
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2).
cortical actin cytoskeleton, cytosol, extracellular exosome, plasma membrane, Z disc
Calculated MW:
103kDa
Observed MW:
103kDa
This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Purification Method:
Affinity purification
Gene ID:
89
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACTN3 Rabbit pAb (CAB12864) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
