The ACTN3 Polyclonal Antibody (CAB12797) is a key tool for researchers studying the ACTN3 gene, which codes for the alpha-actinin-3 protein found in fast-twitch muscle fibers. This antibody, generated in rabbits, displays strong reactivity towards human samples and has been thoroughly validated for use in Western blot applications. By specifically binding to the ACTN3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a valuable asset for investigations in the fields of exercise physiology, sports science, and muscle biology.ACTN3 is known for its role in muscle contraction, particularly in activities requiring explosive power such as sprinting and weightlifting.
Research on ACTN3 can provide insights into athletic performance, muscle function, and the genetic basis of physical abilities. By understanding the functions and variations of ACTN3, scientists and clinicians can further explore its implications in sports performance, exercise training, and even muscle-related disorders. The ACTN3 Polyclonal Antibody is an essential tool for unraveling the complexities of the ACTN3 gene and its impact on human physiology.
Product Name:
ACTN3 Rabbit Polyclonal Antibody
SKU:
CAB12797
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2).
cortical actin cytoskeleton, cytosol, extracellular exosome, plasma membrane, Z disc
Calculated MW:
103kDa
Observed MW:
103kDa
This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Purification Method:
Affinity purification
Gene ID:
89
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACTN3 Rabbit pAb (CAB12797) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
