ACTN2/ACTN3 Antibody (PACO23739)
- SKU:
- PACO23739
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
ACTN2/ACTN3 Antibody (PACO23739)
The ACTN2/ACTN3 Polyclonal Antibody (PAC023739) is a valuable tool for researchers studying actin-binding proteins and their role in muscle function. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting both ACTN2 and ACTN3 proteins in human samples. Validated for use in Western blot applications, this antibody allows for the precise identification and analysis of these important proteins in various cell types.ACTN2 and ACTN3 are actin-binding proteins that play essential roles in muscle structure and function. ACTN2 is predominantly expressed in cardiac muscle, while ACTN3 is primarily found in fast-twitch skeletal muscle fibers. Research suggests that variations in the ACTN3 gene may influence athletic performance and muscle function.
Understanding the expression and function of these proteins is crucial for advancing our knowledge of muscle physiology and related conditions such as muscle disorders and neuromuscular diseases.By utilizing the ACTN2/ACTN3 Polyclonal Antibody, researchers can further investigate the intricate mechanisms underlying muscle biology and development. This antibody offers a reliable tool for exploring the roles of ACTN2 and ACTN3 in various physiological and pathological conditions, paving the way for potential therapeutic interventions and advancements in the field of muscle research.
| Antibody Name: | ACTN2/ACTN3 Antibody (PACO23739) |
| Antibody SKU: | PACO23739 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from N-terminal of human Actin alpha -2/3. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from HeLa cells, HepG2 cells and HUVEC cells, using Actin alpha -2/3 antibody. |
 | Immunofluorescence analysis of HeLa cells, using Actin alpha -2/3 antibody. |
 | Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Actin alpha -2/3 antibody. |
| Background: | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| Synonyms: | Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN2; |
| UniProt Protein Function: | ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 1q42-q43 Cellular Component: cortical actin cytoskeleton; cytoskeleton; focal adhesion; extracellular region; dendritic spine; actin filament; pseudopodium; Z disc; cytosol; filopodium Molecular Function:actin filament binding; protein dimerization activity; integrin binding; identical protein binding; protein binding; LIM domain binding; structural constituent of muscle; cytoskeletal protein binding; titin binding; calcium ion binding; FATZ binding; thyroid hormone receptor coactivator activity Biological Process: focal adhesion formation; platelet activation; negative regulation of potassium ion transport; positive regulation of potassium ion transport; muscle filament sliding; protein homotetramerization; regulation of apoptosis; synaptic transmission; regulation of membrane potential; platelet degranulation; microspike biogenesis; blood coagulation; cell adhesion Disease: Cardiomyopathy, Dilated, 1aa |
| NCBI Summary: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013] |
| UniProt Code: | P35609 |
| NCBI GenInfo Identifier: | 543742 |
| NCBI Gene ID: | 88 |
| NCBI Accession: | P35609.1 |
| UniProt Secondary Accession: | P35609,Q86TF4, Q86TI8, B1ANE4, B2RCS5, |
| UniProt Related Accession: | P35609 |
| Molecular Weight: | 103,920 Da |
| NCBI Full Name: | Alpha-actinin-2 |
| NCBI Synonym Full Names: | actinin, alpha 2 |
| NCBI Official Symbol: | ACTN2Â Â |
| NCBI Official Synonym Symbols: | CMD1AAÂ Â |
| NCBI Protein Information: | alpha-actinin-2; F-actin cross-linking protein; alpha-actinin skeletal muscle |
| UniProt Protein Name: | Alpha-actinin-2 |
| UniProt Synonym Protein Names: | Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein |
| Protein Family: | Alpha-actinin |
| UniProt Gene Name: | ACTN2Â Â |
| UniProt Entry Name: | ACTN2_HUMAN |
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