ACTN1/ACTN2/ACTN3/ACTN4 Antibody (PACO22008)
- SKU:
- PACO22008
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
ACTN1/ACTN2/ACTN3/ACTN4 Antibody (PACO22008)
The ACTN1-4 Polyclonal Antibody (PACO22008) is a versatile tool for researchers studying the actinin family of proteins, specifically ACTN1, ACTN2, ACTN3, and ACTN4. These proteins play key roles in cell structure, adhesion, and motility, making them important targets in the fields of cell biology and cancer research. Raised in rabbits, this antibody demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ACTN1-4 proteins, researchers can effectively detect and analyze these proteins in a variety of cell types, providing valuable insights into their functions and potential implications in disease processes.
The actinins are involved in a wide range of cellular processes, including cell migration, adhesion, and signaling pathways. Dysregulation of these proteins has been linked to various diseases, including cancer, muscular dystrophies, and cardiovascular disorders. By investigating the expression and activity of ACTN1-4, researchers can gain a better understanding of the underlying mechanisms driving these diseases and potentially identify new therapeutic targets for intervention.
| Antibody Name: | ACTN1/ACTN2/ACTN3/ACTN4 Antibody (PACO22008) |
| Antibody SKU: | PACO22008 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from internal of human ACTN4. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from 293 cells, HeLa cells and A549 cells, using ACTN4 antibody. |
| Background: | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| Synonyms: | AAC4; Alpha-actinin 4; F-actin cross linking protein; Non-muscle alpha-actinin 4; actinin |
| UniProt Protein Function: | ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 14q24|14q22-q24 Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; extracellular region; dendritic spine; fascia adherens; pseudopodium; cytosol; Z disc; ruffle; cell projection; cytoplasm; plasma membrane; stress fiber; nucleus Molecular Function:actin filament binding; integrin binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding Biological Process: regulation of apoptosis; actin crosslink formation; focal adhesion formation; platelet activation; actin filament bundle formation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation Disease: Bleeding Disorder, Platelet-type, 15 |
| NCBI Summary: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P12814 |
| NCBI GenInfo Identifier: | 46397817 |
| NCBI Gene ID: | 87 |
| NCBI Accession: | P12814.2 |
| UniProt Secondary Accession: | P12814,Q1HE25, Q9BTN1, B3V8S3, B4DHH3, B7TY16, |
| UniProt Related Accession: | P12814 |
| Molecular Weight: | 892 |
| NCBI Full Name: | Alpha-actinin-1 |
| NCBI Synonym Full Names: | actinin, alpha 1 |
| NCBI Official Symbol: | ACTN1Â Â |
| NCBI Official Synonym Symbols: | BDPLT15Â Â |
| NCBI Protein Information: | alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform |
| UniProt Protein Name: | Alpha-actinin-1 |
| UniProt Synonym Protein Names: | Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1 |
| Protein Family: | Alpha-actinin |
| UniProt Gene Name: | ACTN1Â Â |
| UniProt Entry Name: | ACTN1_HUMAN |
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