The ACTL6A Polyclonal Antibody (CAB5387) is a valuable tool for researchers studying ACTL6A, a key protein involved in chromatin remodeling and transcriptional regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to the ACTL6A protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in molecular biology and gene expression research.ACTL6A, also known as BAF53a, is a subunit of the SWI/SNF chromatin remodeling complex and plays a crucial role in gene regulation by facilitating chromatin accessibility and transcription factor binding.
Dysregulation of ACTL6A has been implicated in various diseases, including cancer and developmental disorders, highlighting its importance in cellular function and gene expression control. Research into the mechanisms underlying ACTL6A activity is essential for gaining insight into disease pathogenesis and developing potential therapeutic interventions targeting this protein.
Product Name:
ACTL6A Rabbit Polyclonal Antibody
SKU:
CAB5387
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-120 of human ACTL6A (NP_004292.1).
This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
86
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ACTL6A Rabbit pAb (CAB5387) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
