Active Caspase-3 Rabbit Monoclonal Antibody (CAB11021)
The Active Caspase-3 Monoclonal Antibody (CAB11021) is a vital tool for researchers studying apoptosis, a crucial process in cell development and homeostasis. Caspase-3 is a key player in the execution phase of apoptosis, responsible for the cleavage of various cellular proteins. This monoclonal antibody, produced in mice, specifically targets active Caspase-3 in various cell types and tissues.With its high specificity and sensitivity, this antibody is ideal for Western blotting and immunohistochemistry applications, enabling accurate detection and analysis of active Caspase-3 levels.
This antibody's ability to specifically recognize active Caspase-3 makes it a valuable asset in research on cell death pathways, cancer biology, and neurodegenerative diseases.Understanding the role of active Caspase-3 in apoptosis is crucial for developing targeted therapies for diseases characterized by abnormal cell death regulation. By providing a reliable tool for detecting and quantifying active Caspase-3, this monoclonal antibody paves the way for further insights into the molecular mechanisms governing apoptosis and potential therapeutic interventions.
Product Name:
Active Caspase-3 Rabbit Monoclonal Antibody
SKU:
CAB11021
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CASP3 (P42574).
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
836
Clone Number:
ARC0185
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using Active Caspase-3 Rabbit mAb (CAB11021) at 1:500 dilution.Jurkat cells were treated by Staurosporine(1 μM) at room temperature for 3 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
